The Department of Pediatrics is seeking candidates for a Biological Scientist II position to perform research in the toxicology core laboratory of the Powell Gene Therapy Center in the Department of Pediatrics. This laboratory follows Good Laboratory Practice (GLP) guidelines as set forth by the US FDA. The incumbent will perform laboratory experiments using gene therapy applications and Standard Operating Procedures (SOPs) established in this lab. Once trained, the individual in this position is expected to function under minimal supervision and troubleshoot simple to moderate complex scientific issues independently (alter or modify SOPs if necessary). The position requires work with rodent animal models and molecular/cellular work.
Responsibilities to include:
- Molecular Techniques and Analysis: Primer design, RNA and DNA isolation, performing real-time PCR and reverse transcriptase real-time PCR, immunoassays to gene therapy vectors and transgenes, including ELISAs and neutralizing antibody assays. This may include expansion/maintenance of cell lines and analysis of transfection/infection. Performing protein analysis by isolating protein of interest from cell or tissue lysates and performing/analyzing western blots. These assays require strict adherence to written SOPs.
- Animal Studies: Will participate in treating animals with pharmacological agents or AAV vectors either through i.p., i.m., or i.v. injection. Other duties with the mice may include doing echocardiography, sensory and motor function, force mechanics, and metabolic studies. Collecting blood and tissues for processing in a clean and sterile manner. Individual will play a key role in regulatory management (IACUCs) and record keeping for each animal using a secure web database.
- Regulatory Compliance: Individual will ensure that laboratory is compliant with governing rules and regulations. Coordinate all IACUC and IRB (exempt) submissions/renewals. Ensure all EH&S standards are met. Training on laboratory SOPS (initially and as they are updated) as well as FDA Good Laboratory Practice (GLP) regulations annually.
- Any other duties, as needed, to fulfill the mission and to abide by the values of the College of Medicine. This will include maintaining and updating laboratory SOP and performing tasks and experiments as directed by the Principal Investigator.
Bachelor’s degree in biology or a closely related field and three years of relevant experience; or a Master’s degree in biology or a closely related field and one year of relevant experience; Doctoral degree in biology or a closely related field.
- Experience in gene therapy research (Specifically AAV).
- Animal colony management and handling of rodents experience.
- Experience maintaining cell lines, performing plasmid transfections/viral infections, immunohistochemistry,
- FACS, rt-PCR, genotyping, immunohistochemistry, western blotting, microscopy
- Advanced degree in related scientific field.
- Working knowledge of basic laboratory techniques.
- Must be extremely knowledgeable in laboratory procedures and must be meticulous in recording, tracking & reporting of data collected.
- Strong interest in translational research.